pKCyR8
(Plasmid
#149494)
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PurposeBroad-Host range expression vector, RK2 and pUC origin, CymR(AM) repressor, PCymRC promoter, mRFP, Hygromycin Resistance Marker.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 149494 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneUnspecified
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Hygromycin, 200 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCymR(AM)-mRFP
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SpeciesSynthetic
- Promoter PCymRC
Cloning Information
- Cloning method Ligation Independent Cloning
Resource Information
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A portion of this plasmid was derived from a plasmid made bysAJM.1506 was received from Addgene from a deposit by Christopher Voight (Addgene plasmid # 108254)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKCyR8 was a gift from Christopher Reisch (Addgene plasmid # 149494 ; http://n2t.net/addgene:149494 ; RRID:Addgene_149494) -
For your References section:
A plasmid toolbox for controlled gene expression across the Proteobacteria. Schuster LA, Reisch CR. Nucleic Acids Res. 2021 Jun 14. pii: 6298615. doi: 10.1093/nar/gkab496. 10.1093/nar/gkab496 PubMed 34125913