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Addgene

pFRG5
(Plasmid #149486)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 149486 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Unspecified
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    RhaS-RhaR-mRFP
  • Species
    Synthetic
  • Promoter PrhaBAD

Cloning Information

  • Cloning method Ligation Independent Cloning

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFRG5 was a gift from Christopher Reisch (Addgene plasmid # 149486 ; http://n2t.net/addgene:149486 ; RRID:Addgene_149486)

  • For your References section:

    A plasmid toolbox for controlled gene expression across the Proteobacteria. Schuster LA, Reisch CR. Nucleic Acids Res. 2021 Jun 14. pii: 6298615. doi: 10.1093/nar/gkab496. 10.1093/nar/gkab496 PubMed 34125913
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