pBRG2
(Plasmid
#149474)
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PurposeBroad-Host range expression vector, pBBR origin, RhaR-RhaS activator, PrhaBAD promoter, GFPmut3b, Kanamycin Resistance Marker.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 149474 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneUnspecified
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameRhaS-RhaR-GFP
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SpeciesSynthetic
- Promoter PrhaBAD
Cloning Information
- Cloning method Ligation Independent Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBRG2 was a gift from Christopher Reisch (Addgene plasmid # 149474 ; http://n2t.net/addgene:149474 ; RRID:Addgene_149474) -
For your References section:
A plasmid toolbox for controlled gene expression across the Proteobacteria. Schuster LA, Reisch CR. Nucleic Acids Res. 2021 Jun 14. pii: 6298615. doi: 10.1093/nar/gkab496. 10.1093/nar/gkab496 PubMed 34125913