pHMGCS-Luc (aka: pES8)
(Plasmid #14947)

• Depositing Lab: Axel Nohturfft
• Publication: Castoreno et al Proc Natl Acad Sci U S A. 2005 Sep 13. 102(37):13129-34.

Backbone

• Vector backbone: pGL2 basic
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 5597
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HMG CoA synthase promoter
• Alt name: 3-hydroxy-3-methylglutaryl CoA synthase
• Alt name: HMGCS
• Species: hamster
• Insert Size (bp): 400
• GenBank ID: AH001829
• Tag / Fusion Protein:
  • luciferase (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: LucNrev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

To generate pHMGCS-Luc, a PCR fragment containing nucleotides –379 to –17 of the hamster 3-hydroxy-3-methylglutaryl (HMG) CoA synthase gene was cloned into pGL2-basic.

PCR'd using primers: CGATCTCCTCTCACTGACCTTC and GCAGCTTTATAGCCACCGACCC.