Skip to main content
Addgene

ratGLUT2
(Plasmid #14946)

Print

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14946 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GLUT2
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    2300
  • Entrez Gene
    Slc2a2 (a.k.a. GTT2, Glut2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

cDNA clone isolated from a rat islet cDNA library constructed in pcDNA-1. The full length insert was excised by KpnI/XbaI and subcloned into pcDNA3.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ratGLUT2 was a gift from Bernard Thorens (Addgene plasmid # 14946 ; http://n2t.net/addgene:14946 ; RRID:Addgene_14946)

  • For your References section:

    Protein kinase A-dependent phosphorylation of GLUT2 in pancreatic beta cells. Thorens B, Deriaz N, Bosco D, DeVos A, Pipeleers D, Schuit F, Meda P, Porret A. J Biol Chem. 1996 Apr 5. 271(14):8075-81. 10.1074/jbc.271.14.8075 PubMed 8626492
Related items:
Commonly requested with: