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Addgene

U6::sgBFP-U6::sgKrasG12D-pX333-red
(Plasmid #149448)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 149448 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    px333
  • Backbone manufacturer
    Addgene #64073
  • Backbone size w/o insert (bp) 9000
  • Total vector size (bp) 5400
  • Modifications to backbone
    Replacement of Cas9 with HcRed
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers
    HcRed

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sgRNA BFP and mouse KRAS G12D
  • gRNA/shRNA sequence
    BFP and mouse KRAS G12D
  • Species
    M. musculus (mouse)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    U6::sgBFP-U6::sgKrasG12D-pX333-red was a gift from Jonathan Chernoff (Addgene plasmid # 149448 ; http://n2t.net/addgene:149448 ; RRID:Addgene_149448)

  • For your References section:

    A Facile Method to Engineer Mutant Kras Alleles in an Isogenic Cell Background. Budagyan K, Chernoff J. Methods Mol Biol. 2021;2262:323-334. doi: 10.1007/978-1-0716-1190-6_20. 10.1007/978-1-0716-1190-6_20 PubMed 33977487
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