Skip to main content
Addgene

pLentiCas9-P2A-BFP
(Plasmid #149447)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 149447 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pLenti-Cas9
  • Backbone size w/o insert (bp) 12364
  • Total vector size (bp) 13177
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    P2A-BFP
  • Alt name
    Blue Fluorescent Protein
  • Insert Size (bp)
    813
  • Promoter In frame with Cas9
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CTGGACGCCACCCTGATCC
  • 3′ sequencing primer TTAAAGCAGCGTATCCACATA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLentiCas9-P2A-BFP was a gift from Jonathan Chernoff (Addgene plasmid # 149447 ; http://n2t.net/addgene:149447 ; RRID:Addgene_149447)

  • For your References section:

    A Facile Method to Engineer Mutant Kras Alleles in an Isogenic Cell Background. Budagyan K, Chernoff J. Methods Mol Biol. 2021;2262:323-334. doi: 10.1007/978-1-0716-1190-6_20. 10.1007/978-1-0716-1190-6_20 PubMed 33977487
Related items:
Commonly requested with: