pET11b-MIF H62A
(Plasmid
#149443)
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PurposeExpression of MIF H62A in E. coli for protein production
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 149443 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET11b
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMIF
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Alt nameMacrophage migration inhibitory factor
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SpeciesH. sapiens (human)
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MutationH62A
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Entrez GeneMIF (a.k.a. GIF, GLIF, MMIF)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
For protein expression, use BL21-Gold (DE3) E. coli
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET11b-MIF H62A was a gift from Elias Lolis (Addgene plasmid # 149443 ; http://n2t.net/addgene:149443 ; RRID:Addgene_149443)