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Addgene

pET11b-MIF Y99A
(Plasmid #149440)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 149440 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET11b
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MIF
  • Alt name
    Macrophage migration inhibitory factor
  • Species
    H. sapiens (human)
  • Mutation
    Y99A
  • Entrez Gene
    MIF (a.k.a. GIF, GLIF, MMIF)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For protein expression, use BL21-Gold (DE3) E. coli

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET11b-MIF Y99A was a gift from Elias Lolis (Addgene plasmid # 149440 ; http://n2t.net/addgene:149440 ; RRID:Addgene_149440)
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