pBigTB
(Plasmid #149432)

• Purpose: (Empty Backbone) shuttle vector with blasticidin-S resistance into ROSA26 targetting vectors
• Depositing Lab: Primo Schaer
• Publication: Schwarz et al F1000Res. 2020 Sep 9;9:1112. doi: 10.12688/f1000research.25637.2. eCollection 2020.

Backbone

• Vector backbone: pBigT (Addgene Plasmid #21270)
• Backbone manufacturer: Costantini Lab
• Backbone size (bp): 4574
• Modifications to backbone: Neomycin resistance casette (SpeI - ClaI) fragment was replaced by blasticidin resistance, amplified by adapter PCR from pcDNA6.2-GW/EmGFP-mir
• Vector type: Mammalian Expression, Mouse Targeting, Cre/Lox
• Promoter: SV40
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: SV40pro-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Supplemental Documents:
  • pBigTB.gbk
• A portion of this plasmid was derived from a plasmid made by: (1) Costantini Lab: pBigT (Addgene plasmid #21270). (2) Invitrogen: blasticidin S-deaminase (BSD) expression cassette amplified from pcDNA6.2-GW/EmGFP-Mir

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry