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Addgene

pTCO2
(Plasmid #149428)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 149428 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript
  • Backbone manufacturer
    Stratagene
  • Backbone size (bp) 3000
  • Modifications to backbone
    multiple cloning steps to generate a mammalian expression vector containing (1) about 6 and 3 kb of the promoter and the 3'UTR/terminator sequences of murine TDG (https://www.ncbi.nlm.nih.gov/gene/21665), respectively, (2) puromycin selection marker controlled by the SV40 promoter/poly(A) signal, and (3) two loxP sites in the Tdg promoter and 3' of the MCS
  • Vector type
    Mammalian Expression, Cre/Lox
  • Promoter murine Tdg promoter
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ sequencing primer M13/pUC forward
  • 3′ sequencing primer M13/pUC reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTCO2 was a gift from Primo Schaer (Addgene plasmid # 149428 ; http://n2t.net/addgene:149428 ; RRID:Addgene_149428)

  • For your References section:

    Inducible TDG knockout models to study epigenetic regulation. Schwarz SD, Grundbacher E, Hrovat AM, Xu J, Kusnierczyk A, Vagbo CB, Schar P, Schuermann D. F1000Res. 2020 Sep 9;9:1112. doi: 10.12688/f1000research.25637.2. eCollection 2020. 10.12688/f1000research.25637.2 PubMed 33082936
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