1098A=TI-pgSIT[sxl,bTub,Hasp70Bb-Cas9]
(Plasmid
#149426)
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PurposeThe attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel sxl and bTub genes, and Hsp70Bb-Cas9-T2A-eGFP-p10.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 149426 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneattB with mini-white
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Vector typeInsect Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameU6.3-gRNAs[Sxl, bTub]
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SpeciesD. melanogaster (fly)
Gene/Insert 2
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Gene/Insert nameHsp70Bb-Cas9-T2A-eGFP-p10
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Alt nameCas9 (Csn1) endonuclease from the Streptococcus pyogenes
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SpeciesD. melanogaster (fly)
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Tag
/ Fusion Protein
- eGFP (C terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
1098A=TI-pgSIT[sxl,bTub,Hasp70Bb-Cas9] was a gift from Omar Akbari (Addgene plasmid # 149426 ; http://n2t.net/addgene:149426 ; RRID:Addgene_149426) -
For your References section:
Temperature-Inducible Precision-Guided Sterile Insect Technique. Kandul NP, Liu J, Akbari OS. CRISPR J. 2021 Dec;4(6):822-835. doi: 10.1089/crispr.2021.0077. Epub 2021 Nov 3. 10.1089/crispr.2021.0077 PubMed 34735288
Map uploaded by the depositor.
