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Addgene

1046E=dgRNAs[traB,bTub]
(Plasmid #149425)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 149425 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    attB with mini-white
  • Vector type
    Insect Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    U6.3-gRNAs[TraB, bTub]
  • gRNA/shRNA sequence
    GATTCCGTACTTTGCAGACGAGG, CCTGAGTGTGCATCAGCTGGTGG
  • Species
    D. melanogaster (fly)
  • Promoter U6.3

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TGTATGCTATACGAAGTTATGCTAGCGGATCCGAATTCTTTTTTGCTCACCTGTGATTGC
  • 3′ sequencing primer ATTTAAATATTGCCCAATTCCCAATTCCCGGTACCAAAAAAAAAAGCACCGACTCGGTGC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    1046E=dgRNAs[traB,bTub] was a gift from Omar Akbari (Addgene plasmid # 149425 ; http://n2t.net/addgene:149425 ; RRID:Addgene_149425)

  • For your References section:

    Temperature-Inducible Precision-Guided Sterile Insect Technique. Kandul NP, Liu J, Akbari OS. CRISPR J. 2021 Dec;4(6):822-835. doi: 10.1089/crispr.2021.0077. Epub 2021 Nov 3. 10.1089/crispr.2021.0077 PubMed 34735288
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