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Addgene

1137=Hsp70Bb-Cas9[dsRed]
(Plasmid #149424)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 149424 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    piggyBac
  • Vector type
    Insect Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cas9
  • Alt name
    Cas9 (Csn1) endonuclease from the Streptococcus pyogenes
  • Species
    Synthetic
  • Promoter Dmel Hsp70Bb
  • Tag / Fusion Protein
    • eGFP (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GGTTCTCGACGGTCACGGCGGGCATGTCGACTCGAGAAATTTCTCTGGCCGTTATTCGTT
  • 3′ sequencing primer CCGTCGTGGTCCTTATAGTCCATTTTGTTTAAACAGAGTTCTCTTCTTGT
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    1137=Hsp70Bb-Cas9[dsRed] was a gift from Omar Akbari (Addgene plasmid # 149424 ; http://n2t.net/addgene:149424 ; RRID:Addgene_149424)

  • For your References section:

    Temperature-Inducible Precision-Guided Sterile Insect Technique. Kandul NP, Liu J, Akbari OS. CRISPR J. 2021 Dec;4(6):822-835. doi: 10.1089/crispr.2021.0077. Epub 2021 Nov 3. 10.1089/crispr.2021.0077 PubMed 34735288
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