pZS*11_4enh
(Plasmid #149423)

• Purpose: Plasmid harboring four plant enhancers (35S, AB80, Cab-1, and rbcS-E9).
• Depositing Lab: Stan Fields
• Publication: Jores et al Plant Cell. 2020 May 14. pii: tpc.20.00155. doi: 10.1105/tpc.20.00155.

Backbone

• Vector backbone: pZS*11-yfp0
• Backbone manufacturer: Subramaniam, Pan, and Cluzel, 2013
• Backbone size w/o insert (bp): 4357
• Total vector size (bp): 5283
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: rbcS-E9 enhancer
• Species: Pisum sativum
• Insert Size (bp): 234
• GenBank ID: X00806

Gene/Insert 2

• Gene/Insert name: 35S core enhancer
• Species: Cauliflower mosaic virus
• Insert Size (bp): 153
• GenBank ID: V00141

Gene/Insert 3

• Gene/Insert name: Cab-1 enhancer
• Species: Triticum aestivum
• Insert Size (bp): 268
• GenBank ID: X05823
• Tag / Fusion Protein:
  • T120>A

Gene/Insert 4

• Gene/Insert name: AB80 enhancer
• Species: Pisum sativum
• Insert Size (bp): 247
• GenBank ID: X03074

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry