pPSint
(Plasmid
#149421)
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Purpose(Empty Backbone) Plasmid for STARR-seq in tobacco leaves. Enhancer candidates can be inserted into the IV2 intron in the GFP reporter gene.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 149421 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGreen
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Backbone manufacturerHellens et al, 2000
- Backbone size (bp) 3232
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Vector typePlant Expression
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Selectable markersPhosphinothricin
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPSint was a gift from Stan Fields (Addgene plasmid # 149421 ; http://n2t.net/addgene:149421 ; RRID:Addgene_149421) -
For your References section:
Identification of Plant Enhancers and Their Constituent Elements by STARR-seq in Tobacco Leaves. Jores T, Tonnies J, Dorrity MW, Cuperus J, Fields S, Queitsch C. Plant Cell. 2020 May 14. pii: tpc.20.00155. doi: 10.1105/tpc.20.00155. 10.1105/tpc.20.00155 PubMed 32409318
