pLDLR-Luc (aka: pES7)
(Plasmid #14940)

• Depositing Lab: Axel Nohturfft
• Publication: Castoreno et al Proc Natl Acad Sci U S A. 2005 Sep 13. 102(37):13129-34.

Backbone

• Vector backbone: pGL2 basic
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 5597
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: LDLR promoter
• Alt name: LDL receptor promoter
• Alt name: LDL receptor
• Species: H. sapiens (human)
• Insert Size (bp): 340
• GenBank ID: NC_000019
• Entrez Gene: LDLR (a.k.a. FH, FHC, FHCL1, LDLCQ2)
• Tag / Fusion Protein:
  • luciferase (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SacI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: LucNrev

Resource Information

• Articles Citing this Plasmid:
  • 11 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

To generate pLDLR-Luc, a PCR fragment containing nucleotides –335 to +3 of the human LDL receptor gene was cloned into pGL2-basic.

PCR'd using primers: CGATCCTCTACTGACCTC and GGCCCACGTCATTTACAGCATTTC.