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Addgene

pCRISPR2_motif_scram
(Plasmid #149397)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 149397 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHERD30T
  • Backbone size w/o insert (bp) 5220
  • Total vector size (bp) 7120
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Pseudomonas aeruginosa PA14 CRISPR2 locus
  • Alt name
    P. aeruginosa PA14 CRISPR locus adjacent to the Cas1 gene
  • Species
    Pseudomonas aeruginosa UCBPP-PA14
  • Insert Size (bp)
    1900
  • Mutation
    Upstream motif recognized by Cas1-2/3 during spacer integration is scrambled
  • Promoter pBAD

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (unknown if destroyed)
  • 3′ cloning site SphI (unknown if destroyed)
  • 5′ sequencing primer pBAD_F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCRISPR2_motif_scram was a gift from Blake Wiedenheft (Addgene plasmid # 149397 ; http://n2t.net/addgene:149397 ; RRID:Addgene_149397)

  • For your References section:

    Distribution and phasing of sequence motifs that facilitate CRISPR adaptation. Santiago-Frangos A, Buyukyoruk M, Wiegand T, Krishna P, Wiedenheft B. Curr Biol. 2021 Aug 23;31(16):3515-3524.e6. doi: 10.1016/j.cub.2021.05.068. Epub 2021 Jun 25. 10.1016/j.cub.2021.05.068 PubMed 34174210
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