CS2 Flag-OAZ
(Plasmid #14939)

• Depositing Lab: Joan Massague
• Publication: Hata et al Cell. 2000 Jan 21. 100(2):229-40.

Backbone

• Vector backbone: pCS2
• Backbone size w/o insert (bp): 4100
• Vector type: xenopus/mammalian/avian/zebrafish

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: OAZ
• Species: H. sapiens (human)
• Entrez Gene: ZNF423 (a.k.a. Ebfaz, JBTS19, NPHP14, OAZ, Roaz, ZFP423, Zfp104, hOAZ)
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: EBV rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

A human OAZ cDNA missing the first 18 codons (KIAA0760) was obtained from the Kazusa DNA Research Institute. A clone containing the amino-terminal end of human OAZ cDNA (ATCC Clone No. 2019478) was obtained from the EST database and sequenced. Full-length cDNA encoding human OAZ was constructed by fusing synthesized oligonucleotides encoding the first 18 amino acids to the KIAA0760 clone. A Flag epitope tag was introduced at the amino terminus of OAZ by PCR.