CS2 Flag-OAZ
(Plasmid
#14939)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 14939 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCS2
- Backbone size w/o insert (bp) 4100
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Vector typexenopus/mammalian/avian/zebrafish
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameOAZ
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SpeciesH. sapiens (human)
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Entrez GeneZNF423 (a.k.a. Ebfaz, JBTS19, NPHP14, OAZ, Roaz, ZFP423, Zfp104, hOAZ)
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Tag
/ Fusion Protein
- Flag (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer EBV rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
A human OAZ cDNA missing the first 18 codons (KIAA0760) was obtained from the Kazusa DNA Research Institute. A clone containing the amino-terminal end of human OAZ cDNA (ATCC Clone No. 2019478) was obtained from the EST database and sequenced. Full-length cDNA encoding human OAZ was constructed by fusing synthesized oligonucleotides encoding the first 18 amino acids to the KIAA0760 clone. A Flag epitope tag was introduced at the amino terminus of OAZ by PCR.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CS2 Flag-OAZ was a gift from Joan Massague (Addgene plasmid # 14939 ; http://n2t.net/addgene:14939 ; RRID:Addgene_14939) -
For your References section:
OAZ uses distinct DNA- and protein-binding zinc fingers in separate BMP-Smad and Olf signaling pathways. Hata A, Seoane J, Lagna G, Montalvo E, Hemmati-Brivanlou A, Massague J. Cell. 2000 Jan 21. 100(2):229-40. 10.1016/S0092-8674(00)81561-5 PubMed 10660046
Map uploaded by the depositor.
![](https://media.addgene.org/data/easy-thumbnails/data/09/69/b6443352-af61-11e0-90fe-003048dd6500.jpeg.940x940_q85_autocrop.png)