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Addgene

pCRISPR2_+1D
(Plasmid #149389)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 149389 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHERD30T
  • Backbone size w/o insert (bp) 5220
  • Total vector size (bp) 7120
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Pseudomonas aeruginosa PA14 CRISPR2 locus
  • Alt name
    P. aeruginosa PA14 CRISPR locus adjacent to the Cas1 gene
  • Species
    Pseudomonas aeruginosa UCBPP-PA14
  • Insert Size (bp)
    1900
  • Mutation
    1bp inserted downstream of the IHFprox site
  • Promoter pBAD

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (unknown if destroyed)
  • 3′ cloning site SphI (unknown if destroyed)
  • 5′ sequencing primer pBAD_F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note there is a 200bp addition that adds 3 28nt_Csyr_rpt regions compared to the deposited sequence. Depositor confirms this does not impact the use of this plasmid for the relevant biochemical assays performed in their paper.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCRISPR2_+1D was a gift from Blake Wiedenheft (Addgene plasmid # 149389 ; http://n2t.net/addgene:149389 ; RRID:Addgene_149389)

  • For your References section:

    Distribution and phasing of sequence motifs that facilitate CRISPR adaptation. Santiago-Frangos A, Buyukyoruk M, Wiegand T, Krishna P, Wiedenheft B. Curr Biol. 2021 Aug 23;31(16):3515-3524.e6. doi: 10.1016/j.cub.2021.05.068. Epub 2021 Jun 25. 10.1016/j.cub.2021.05.068 PubMed 34174210
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