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Addgene

pStrepIHFb
(Plasmid #149385)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 149385 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5369
  • Total vector size (bp) 5588
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Pseudomonas aeruginosa PA14 StrepII-tagged IHFbeta
  • Species
    Pseudomonas aeruginosa UCBPP-PA14
  • Insert Size (bp)
    340
  • GenBank ID
    WP_003091441.1
  • Promoter T7
  • Tags / Fusion Proteins
    • StrepII-tag with (N terminal on insert)
    • HRV3C cleavage site (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (unknown if destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)
  • 5′ sequencing primer T7 Fwd
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pStrepIHFb was a gift from Blake Wiedenheft (Addgene plasmid # 149385 ; http://n2t.net/addgene:149385 ; RRID:Addgene_149385)

  • For your References section:

    Distribution and phasing of sequence motifs that facilitate CRISPR adaptation. Santiago-Frangos A, Buyukyoruk M, Wiegand T, Krishna P, Wiedenheft B. Curr Biol. 2021 Aug 23;31(16):3515-3524.e6. doi: 10.1016/j.cub.2021.05.068. Epub 2021 Jun 25. 10.1016/j.cub.2021.05.068 PubMed 34174210
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