pHisIHFαIHFβ
(Plasmid #149384)

• Purpose: Expression of P. aeruginosa PA14, 6xHis-HRV3C-IHFalpha and IHFbeta
• Depositing Lab: Blake Wiedenheft
• Publication: Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.e6. doi: 10.1016/j.cub.2021.05.068. Epub 2021 Jun 25.

Backbone

• Vector backbone: pACYCDuet-1
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 4008
• Total vector size (bp): 4468
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: Pseudomonas aeruginosa PA14 6xHis-tagged IHFalpha
• Species: Pseudomonas aeruginosa UCBPP-PA14
• Insert Size (bp): 370
• GenBank ID: WP_003090661.1
• Promoter: T7
• Tags / Fusion Proteins:
  • 6xHis tag (N terminal on insert)
  • HRV3C cleavage site (N terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: HindIII (unknown if destroyed)
• 5′ sequencing primer: DuetUP1

Gene/Insert 2

• Gene/Insert name: Pseudomonas aeruginosa PA14 IHFbeta
• Species: Pseudomonas aeruginosa UCBPP-PA14
• Insert Size (bp): 290
• GenBank ID: WP_003091441.1
• Promoter: T7

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (unknown if destroyed)
• 3′ cloning site: AvrII (unknown if destroyed)
• 5′ sequencing primer: DuetUP2

Resource Information

• Supplemental Documents:
  • pHisIHFαIHFβ.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry