Flag FoxO3 NT
(Plasmid
#14938)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 14938 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneFlag-pCMV5
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFoxO3 NT
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Alt nameFoxO3 N-terminus
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Alt nameFoxO3
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SpeciesH. sapiens (human)
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Insert Size (bp)750
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Mutationaa 1-249.
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Entrez GeneFOXO3 (a.k.a. AF6q21, FKHRL1, FKHRL1P2, FOXO2, FOXO3A)
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Tag
/ Fusion Protein
- Flag (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer CMV Forward (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Flag FoxO3 NT was a gift from Joan Massague (Addgene plasmid # 14938 ; http://n2t.net/addgene:14938 ; RRID:Addgene_14938) -
For your References section:
Integration of Smad and forkhead pathways in the control of neuroepithelial and glioblastoma cell proliferation. Seoane J, Le HV, Shen L, Anderson SA, Massague J. Cell. 2004 Apr 16. 117(2):211-23. 10.1016/S0092-8674(04)00298-3 PubMed 15084259
Map uploaded by the depositor.
