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Addgene

mUAVbla:tetR 5′
(Plasmid #149379)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 149379 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    mUAVbla
  • Backbone size w/o insert (bp) 3101
  • Total vector size (bp) 3671
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Tetracycline, 100 & 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    tetA
  • Alt name
    tetR
  • Species
    Synthetic; E. coli

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AarI (destroyed during cloning)
  • 3′ cloning site AarI (destroyed during cloning)
  • 5′ sequencing primer AATAGGCGTATCACGAGGC
  • 3′ sequencing primer AGCGAGTCAGTGAGCGAG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mUAVbla:tetR 5′ was a gift from Mark Pallen (Addgene plasmid # 149379 ; http://n2t.net/addgene:149379 ; RRID:Addgene_149379)

  • For your References section:

    Creation of Golden Gate constructs for gene doctoring. Thomson NM, Zhang C, Trampari E, Pallen MJ. BMC Biotechnol. 2020 Oct 7;20(1):54. doi: 10.1186/s12896-020-00648-5. 10.1186/s12896-020-00648-5 PubMed 33028286
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