pAAV-FLEX-tTA
(Plasmid
#149363)
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PurposeAAV-mediated and Cre-dependent expression of tTA under the CAG promoter.
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 149363 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneAAV-FLEX
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Vector typeMammalian Expression, AAV, Cre/Lox
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nametTA2
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SpeciesE. coli
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Insert Size (bp)747
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer cttctggcgtgtgaccgg (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byThe tTA2 gene derived from pTet-Off Advanced vector (Clontech).
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2022.10.20.512984v1 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-FLEX-tTA was a gift from Takeshi Imai (Addgene plasmid # 149363 ; http://n2t.net/addgene:149363 ; RRID:Addgene_149363) -
For your References section:
Automated neuronal reconstruction with super-multicolour fluorescence imaging. Leiwe MN, Fujimoto S, Baba T, Moriyasu D, Saha B, Sakaguchi R, Inagaki S, Imai T. bioRxiv 2022.10.20.512984 10.1101/2022.10.20.512984
