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Addgene

lenti-CDDR
(Plasmid #149355)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 149355 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCW-Cas9
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    CDDR Reporter
  • Alt name
    CRISPR/Cas9-based Dual-fluorescent DSB Repair Reporter
  • Insert Size (bp)
    1487
  • Promoter EF-1a

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    Blasticidin resistance
  • Alt name
    BSD
  • Insert Size (bp)
    396
  • Promoter hPGK

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site XmaI (not destroyed)
  • 5′ sequencing primer cactagtaccctcgcagacg
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    lenti-CDDR was a gift from Tarek Abbas (Addgene plasmid # 149355 ; http://n2t.net/addgene:149355 ; RRID:Addgene_149355)

  • For your References section:

    A robust CRISPR-Cas9-based fluorescent reporter assay for the detection and quantification of DNA double-strand break repair. Eki R, She J, Parlak M, Benamar M, Du KP, Kumar P, Abbas T. Nucleic Acids Res. 2020 Oct 17. pii: 5929239. doi: 10.1093/nar/gkaa897. 10.1093/nar/gkaa897 PubMed 33068408
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