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pCbh-SpyCas9-T2A-puro-pA_U6-sgPolb (pE047)
(Plasmid #149350)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 149350 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    puc19
  • Backbone size w/o insert (bp) 2704
  • Total vector size (bp) 9346
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SpyCas9
  • Species
    Streptococcus pyogenes
  • Insert Size (bp)
    4269
  • Promoter Cbh
  • Tag / Fusion Protein
    • Flag tag

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer M13 for
  • 3′ sequencing primer M13 rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCbh-SpyCas9-T2A-puro-pA_U6-sgPolb (pE047) was a gift from Ralf Kuehn (Addgene plasmid # 149350 ; http://n2t.net/addgene:149350 ; RRID:Addgene_149350)

  • For your References section:

    A homology independent sequence replacement strategy in human cells using a CRISPR nuclease. Danner E, Lebedin M, de la Rosa K, Kuhn R. Open Biol. 2021 Jan;11(1):200283. doi: 10.1098/rsob.200283. Epub 2021 Jan 27. 10.1098/rsob.200283 PubMed 33499763
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