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Addgene

pMC_cherry donor_Polb target (pE038)
(Plasmid #149349)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 149349 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    puc19
  • Backbone size w/o insert (bp) 3225
  • Total vector size (bp) 4841

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cherry
  • Insert Size (bp)
    711

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMC_cherry donor_Polb target (pE038) was a gift from Ralf Kuehn (Addgene plasmid # 149349 ; http://n2t.net/addgene:149349 ; RRID:Addgene_149349)

  • For your References section:

    A homology independent sequence replacement strategy in human cells using a CRISPR nuclease. Danner E, Lebedin M, de la Rosa K, Kuhn R. Open Biol. 2021 Jan;11(1):200283. doi: 10.1098/rsob.200283. Epub 2021 Jan 27. 10.1098/rsob.200283 PubMed 33499763
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