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Addgene

Prham-tagRFP-IMBB2.4-pUC57-mini
(Plasmid #149343)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 149343 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC
  • Backbone manufacturer
    GenScript
  • Backbone size w/o insert (bp) 1632
  • Total vector size (bp) 2939
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Please use Mach1 bacterial strain for expression.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    tagRFP
  • Alt name
    Red Fluorescent Protein
  • Species
    Synthetic; Entacmaea quadricolor
  • Insert Size (bp)
    694
  • GenBank ID
    KJ908188
  • Promoter rhamnose

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site SpeI (not destroyed)
  • 5′ sequencing primer part sequenced in different plasmid prior to subcloning
  • 3′ sequencing primer part sequenced in different plasmid prior to subcloning
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Prham-tagRFP-IMBB2.4-pUC57-mini was a gift from Ichiro Matsumura (Addgene plasmid # 149343 ; http://n2t.net/addgene:149343 ; RRID:Addgene_149343)

  • For your References section:

    Methylase-assisted subcloning for high throughput BioBrick assembly. Matsumura I. PeerJ. 2020 Sep 11;8:e9841. doi: 10.7717/peerj.9841. eCollection 2020. 10.7717/peerj.9841 PubMed 32974095
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