Prham-M.EcoRI-p15A-aadA
(Plasmid
#149341)
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Purposeexpresses M.EcoRI DNA methyltransferase in E. coli
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 149341 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepACYC
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Backbone manufacturerEMD Millipore/Novagen
- Backbone size w/o insert (bp) 1724
- Total vector size (bp) 3352
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsPlease use OmniMax 2 bacterial strain for expression.
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameM.EcoRI
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Alt nameEcoRI DNA methyltransferase
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SpeciesSynthetic; Plasmid RI13 (from E.coli)
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GenBank IDJ01675
- Promoter rhamnose
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (destroyed during cloning)
- 3′ cloning site SpeI (destroyed during cloning)
- 5′ sequencing primer part sequenced in different plasmid prior to subcloning
- 3′ sequencing primer part sequenced in different plasmid prior to subcloning (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Prham-M.EcoRI-p15A-aadA was a gift from Ichiro Matsumura (Addgene plasmid # 149341 ; http://n2t.net/addgene:149341 ; RRID:Addgene_149341) -
For your References section:
Methylase-assisted subcloning for high throughput BioBrick assembly. Matsumura I. PeerJ. 2020 Sep 11;8:e9841. doi: 10.7717/peerj.9841. eCollection 2020. 10.7717/peerj.9841 PubMed 32974095