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Addgene

Prham-M.PstI-p15A-aadA
(Plasmid #149340)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 149340 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pACYC
  • Backbone manufacturer
    EMD Milliipore/Novagen
  • Backbone size w/o insert (bp) 1724
  • Total vector size (bp) 3883
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Please use OmniMax 2 bacterial strain for expression.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    M.,PstI
  • Alt name
    PstI DNA methyltransferase
  • Species
    Synthetic; Providencia stuartii 164
  • Insert Size (bp)
    1523
  • GenBank ID
    K02081
  • Promoter rhamnose

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (destroyed during cloning)
  • 3′ cloning site SpeI (destroyed during cloning)
  • 5′ sequencing primer part sequenced in different plasmid prior to subcloning
  • 3′ sequencing primer part sequenced in different plasmid prior to subcloning
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The wild-type M.PstI DNA methyltransferase expressed by this plasmid is suitable for 4R/2M BioBrick assembly, unlike the R214H mutant that was described in the PeerJ study but discovered only after its publication.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Prham-M.PstI-p15A-aadA was a gift from Ichiro Matsumura (Addgene plasmid # 149340 ; http://n2t.net/addgene:149340 ; RRID:Addgene_149340)

  • For your References section:

    Methylase-assisted subcloning for high throughput BioBrick assembly. Matsumura I. PeerJ. 2020 Sep 11;8:e9841. doi: 10.7717/peerj.9841. eCollection 2020. 10.7717/peerj.9841 PubMed 32974095
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