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Addgene

pTP396
(Plasmid #149336)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 149336 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDG-Kan
  • Backbone manufacturer
    Görlich lab
  • Backbone size w/o insert (bp) 4483
  • Total vector size (bp) 4831
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    anti-GFP nanobody
  • Alt name
    Enhancer
  • Species
    Llama glama
  • Insert Size (bp)
    348
  • Tags / Fusion Proteins
    • His14 (N terminal on insert)
    • Biotin acceptor peptide (Avi-tag) (N terminal on insert)
    • SUMOEu1 cleavage module (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (unknown if destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)
  • 5′ sequencing primer pQE-for
  • 3′ sequencing primer pQE-rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTP396 was a gift from Rebecca Voorhees (Addgene plasmid # 149336 ; http://n2t.net/addgene:149336 ; RRID:Addgene_149336)

  • For your References section:

    Structural basis for membrane insertion by the human ER membrane protein complex. Pleiner T, Pinton Tomaleri G, Januszyk K, Inglis AJ, Hazu M, Voorhees RM. Science. 2020 May 21. pii: science.abb5008. doi: 10.1126/science.abb5008. 10.1126/science.abb5008 PubMed 32439656
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