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pTP264
(Plasmid #149334)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 149334 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDG-Kan
  • Backbone manufacturer
    Görlich lab
  • Backbone size w/o insert (bp) 4278
  • Total vector size (bp) 5243
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Biotin ligase BirA
  • Species
    E. coli
  • Insert Size (bp)
    965
  • Tags / Fusion Proteins
    • His14 (N terminal on insert)
    • bdNEDD8 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (unknown if destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)
  • 5′ sequencing primer pQE-for
  • 3′ sequencing primer pQE-rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTP264 was a gift from Dirk Görlich (Addgene plasmid # 149334 ; http://n2t.net/addgene:149334 ; RRID:Addgene_149334)

  • For your References section:

    Nanobodies: site-specific labeling for super-resolution imaging, rapid epitope-mapping and native protein complex isolation. Pleiner T, Bates M, Trakhanov S, Lee CT, Schliep JE, Chug H, Bohning M, Stark H, Urlaub H, Gorlich D. Elife. 2015 Dec 3;4:e11349. doi: 10.7554/eLife.11349. 10.7554/eLife.11349 PubMed 26633879
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