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PurposeBacterial expression of SENP_EuB protease
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 149333 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDG-Kan
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Backbone manufacturerGörlich lab
- Backbone size w/o insert (bp) 3997
- Total vector size (bp) 4720
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSENP_EuB protease
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SpeciesSynthetic
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Insert Size (bp)723
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Tags
/ Fusion Proteins
- His14 (N terminal on insert)
- Tev protease cleavage site (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (unknown if destroyed)
- 3′ cloning site HindIII (unknown if destroyed)
- 5′ sequencing primer pQE-for
- 3′ sequencing primer pQE-rev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAV0286 was a gift from Dirk Görlich (Addgene plasmid # 149333 ; http://n2t.net/addgene:149333 ; RRID:Addgene_149333) -
For your References section:
Engineered SUMO/protease system identifies Pdr6 as a bidirectional nuclear transport receptor. Vera Rodriguez A, Frey S, Gorlich D. J Cell Biol. 2019 Jun 3;218(6):2006-2020. doi: 10.1083/jcb.201812091. Epub 2019 Apr 25. 10.1083/jcb.201812091 PubMed 31023724
