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Addgene

pCMV5 Flag-NLS-Smad2
(Plasmid #14931)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14931 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV5
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NLS-Smad2
  • Alt name
    Smad2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1400
  • Mutation
    full-length Smad2 is fused to a nuclear-localization sequence (NLS)
  • Entrez Gene
    SMAD2 (a.k.a. CHTD8, JV18, JV18-1, LDS6, MADH2, MADR2, hMAD-2, hSMAD2)
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV Forward
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV5 Flag-NLS-Smad2 was a gift from Joan Massague (Addgene plasmid # 14931 ; http://n2t.net/addgene:14931 ; RRID:Addgene_14931)

  • For your References section:

    Ubiquitin-dependent degradation of TGF-beta-activated smad2. Lo RS, Massague J. Nat Cell Biol. 1999 Dec . 1(8):472-8. 10.1038/70258 PubMed 10587642
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