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pAAV-nef-CIAO2-TVA-mCherry
(Plasmid #149297)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 149297 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV-nef-CIAO2
  • Backbone size w/o insert (bp) 5383
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TVA:mCherry
  • Species
    G. gallus (chicken)
  • Insert Size (bp)
    1149
  • Mutation
    Start codon removed.

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The CIAO2 construct replaces the lox66/71 pair used in CIAO with the lox43/44 pair to further decrease leak expression - not used in the original publication. Full name: pAAV-nef-AO-lox43-STOP-lox44-TVA:mCherry-hGH-pA.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-nef-CIAO2-TVA-mCherry was a gift from Edward Callaway (Addgene plasmid # 149297 ; http://n2t.net/addgene:149297 ; RRID:Addgene_149297)

  • For your References section:

    Sources of off-target expression from recombinase-dependent AAV vectors and mitigation with cross-over insensitive ATG-out vectors. Fischer KB, Collins HK, Callaway EM. Proc Natl Acad Sci U S A. 2019 Dec 16. pii: 1915974116. doi: 10.1073/pnas.1915974116. 10.1073/pnas.1915974116 PubMed 31843925
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