Skip to main content
Addgene

pAAV-nef-CIAO-Flp
(Plasmid #149294)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 149294 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pAAV-nef-CIAO
  • Backbone size w/o insert (bp) 5383
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Flp recombinase
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1275
  • Mutation
    Derived from the mouse codon-optomized FlpO. The SV40 NLS signal first start codon have been removed.
  • Entrez Gene
    flp

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Full name: pAAV-nef-AO-lox66-STOP-lox71-Flp-hGH-pA

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-nef-CIAO-Flp was a gift from Edward Callaway (Addgene plasmid # 149294 ; http://n2t.net/addgene:149294 ; RRID:Addgene_149294)

  • For your References section:

    Sources of off-target expression from recombinase-dependent AAV vectors and mitigation with cross-over insensitive ATG-out vectors. Fischer KB, Collins HK, Callaway EM. Proc Natl Acad Sci U S A. 2019 Dec 16. pii: 1915974116. doi: 10.1073/pnas.1915974116. 10.1073/pnas.1915974116 PubMed 31843925
Related items:
Commonly requested with: