SPDK3876(TRV-RNA2-pPEBV-MCS)
(Plasmid #149275)

• Purpose: Modified Tobacco rattle virus RNA2 vector that could be used for expression of proteins or RNAs
• Depositing Lab: Savithramma Dinesh-Kumar
• Publication: Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41477-020-0670-y. doi: 10.1038/s41477-020-0670-y.

Backbone

• Vector backbone: pCAMBIA0390 T-DNA vector
• Backbone size w/o insert (bp): 6426
• Total vector size (bp): 9587
• Vector type: T-DNA vector

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: We prefer to use DH10B cells
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Modified TRV RNA2 with PEBV promoter
• Species: Modified Tobacco Rattle Virus RNA 2 with PEBV promoter
• Insert Size (bp): 2030
• Mutation: T2318C (DNA) in PEBV coat protein sequence

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI, StuI, BamHI, KpnI, SacI, MluI, XhoI, XmaI/SmaI (unknown if destroyed)
• 3′ cloning site: None (unknown if destroyed)
• 5′ sequencing primer: CATAATTATACTGATTTGTCTCTCG

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry