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Addgene

PA RdgC_pET21a
(Plasmid #14927)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14927 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-21a(+)
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5443
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Recombination-associated protein rdgC
  • Alt name
    RdgC
  • Species
    Pseudomonas aeruginosa
  • Insert Size (bp)
    918
  • GenBank ID
    Q9HYX7
  • Tag / Fusion Protein
    • His (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PA RdgC_pET21a was a gift from SeWon Suh (Addgene plasmid # 14927 ; http://n2t.net/addgene:14927 ; RRID:Addgene_14927)

  • For your References section:

    The recombination-associated protein RdgC adopts a novel toroidal architecture for DNA binding. Ha JY, Kim HK, Kim DJ, Kim KH, Oh SJ, Lee HH, Yoon HJ, Song HK, Suh SW. Nucleic Acids Res. 2007 Apr 10. ():. 10.1093/nar/gkm144 PubMed 17426134
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