pGBW-m4046800
(Plasmid #149235)

• Purpose: Bacterial expression plasmid for SARS-CoV-2 ORF3a protein
• Depositing Labs: Ginkgo Bioworks, Benjie Chen
• Publication: SARS-CoV-2 protein E. coli expression constructs, tagged, for protein expression and/or purification (unpublished)

Backbone

• Vector backbone: ori-013 - marker | cmR
• Backbone size w/o insert (bp): 1462
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SARS-CoV-2 ORF3a protein
• Species: Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2
• Insert Size (bp): 821
• Mutation: Escherichia coli recode 1
• Entrez Gene: ORF3a (a.k.a. GU280_gp03)
• Promoter: Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ; Start Codon | ATG
• Tag / Fusion Protein:
  • Cleavable TEV;6xHIS (C terminal on backbone)

Cloning Information

• Cloning method: Unknown

Resource Information

• A portion of this plasmid was derived from a plasmid made by: GinkgoBioworks

Terms and Licenses

• Academic/Nonprofit Terms:
  • OpenMTA
• Industry Terms:
  • OpenMTA

Depositor Comments

NCBI Reference Sequence: YP_009724391.1 NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid.