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Addgene

pRSETB PKA RIalpha
(Plasmid #14922)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14922 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRSET B
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PKA R1alpha regulatory subunit
  • Alt name
    PKA
  • Alt name
    PRKAR1A
  • Species
    B. taurus (bovine)
  • Entrez Gene
    PRKAR1A

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSETB PKA RIalpha was a gift from Susan Taylor (Addgene plasmid # 14922 ; http://n2t.net/addgene:14922 ; RRID:Addgene_14922)

  • For your References section:

    Dynamics of signaling by PKA. Taylor SS, Kim C, Vigil D, Haste NM, Yang J, Wu J, Anand GS. Biochim Biophys Acta. 2005 Dec 30. 1754(1-2):25-37. 10.1016/j.bbapap.2005.08.024 PubMed 16214430
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