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Addgene

pET15b PKA Cat
(Plasmid #14921)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14921 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET15b
  • Backbone manufacturer
    EMD Biosciences
  • Backbone size w/o insert (bp) 5700
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PKA catalytic subunit alpha
  • Alt name
    PKA
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Prkaca (a.k.a. PKCD, Pkaca)
  • Tags / Fusion Proteins
    • 6xHis (N terminal on backbone)
    • Thrombin cleavage site (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET15b PKA Cat was a gift from Susan Taylor (Addgene plasmid # 14921 ; http://n2t.net/addgene:14921 ; RRID:Addgene_14921)

  • For your References section:

    Crystal structure of a polyhistidine-tagged recombinant catalytic subunit of cAMP-dependent protein kinase complexed with the peptide inhibitor PKI(5-24) and adenosine. Narayana N, Cox S, Shaltiel S, Taylor SS, Xuong N. Biochemistry. 1997 Apr 15. 36(15):4438-48. 10.1021/bi961947+ PubMed 9109651
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