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Addgene

pRSETB PKA Cat
(Plasmid #14920)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14920 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRSET B
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PKA catalytic subunit alpha
  • Alt name
    PKA
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Prkaca (a.k.a. PKCD, Pkaca)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSETB PKA Cat was a gift from Susan Taylor (Addgene plasmid # 14920 ; http://n2t.net/addgene:14920 ; RRID:Addgene_14920)

  • For your References section:

    Dynamics of signaling by PKA. Taylor SS, Kim C, Vigil D, Haste NM, Yang J, Wu J, Anand GS. Biochim Biophys Acta. 2005 Dec 30. 1754(1-2):25-37. 10.1016/j.bbapap.2005.08.024 PubMed 16214430
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