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Addgene

pAJ20
(Plasmid #14919)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14919 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAJ04
  • Backbone size w/o insert (bp) 4224
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Strongyloides stercoralis Ribosomal protein small subunit
  • Alt name
    Ss rps-21
  • Species
    Strongyloides stercoralis
  • Insert Size (bp)
    3301
  • GenBank ID
    EF589665
  • Tag / Fusion Protein
    • GFP(S65C) (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site AgeI (not destroyed)
  • 5′ sequencing primer M13 forward
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

derived from pAJ04.
See Fire Lab C. elegans Vector Kit 1995 Documetation.

PAPER:Int J Parasitol. 2006 May 31;36(6):671-9. Epub 2006 Feb 7 Successful transgenesis of the parasitic nematode Strongyloides stercoralis requires endogenous non-coding control elements.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAJ20 was a gift from James Lok (Addgene plasmid # 14919 ; http://n2t.net/addgene:14919 ; RRID:Addgene_14919)

  • For your References section:

    Strongyloides stercoralis: cell- and tissue-specific transgene expression and co-transformation with vector constructs incorporating a common multifunctional 3' UTR. Junio AB, Li X, Massey HC, Nolan TJ, Todd Lamitina S, Sundaram MV, Lok JB. Exp Parasitol. 2008 Feb . 118(2):253-65. 10.1016/j.exppara.2007.08.018 PubMed 17945217
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