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Addgene

pAJ50
(Plasmid #14918)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14918 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAJ08
  • Backbone size w/o insert (bp) 4515
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    enhanced monomeric red fluorescent protein
  • Alt name
    mRFPmars
  • Alt name
    synthetic RFP
  • Species
    Dictyostelium discoideum
  • Insert Size (bp)
    682
  • GenBank ID
    AY679163

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site AvrII (not destroyed)
  • 5′ sequencing primer M13
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Provided in by Dr. Jakob Franke at the Dicty Stock Center, Columbia University (http://dictybase.org/stockcenter/stockcenter.html). Paper: FEBS Letters 577 (2004) 227–232. A brilliant monomeric red fluorescent protein to visualize cytoskeleton dynamics in Dictyostelium.
  • Article Citing this Plasmid

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

PAPER:Int J Parasitol. 2006 May 31;36(6):671-9. Epub 2006 Feb 7 Successful transgenesis of the parasitic nematode Strongyloides stercoralis requires endogenous non-coding control elements.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAJ50 was a gift from James Lok (Addgene plasmid # 14918 ; http://n2t.net/addgene:14918 ; RRID:Addgene_14918)

  • For your References section:

    Strongyloides stercoralis: cell- and tissue-specific transgene expression and co-transformation with vector constructs incorporating a common multifunctional 3' UTR. Junio AB, Li X, Massey HC, Nolan TJ, Todd Lamitina S, Sundaram MV, Lok JB. Exp Parasitol. 2008 Feb . 118(2):253-65. 10.1016/j.exppara.2007.08.018 PubMed 17945217
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