pAJ15
(Plasmid
#14917)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 14917 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAJ04
- Backbone size w/o insert (bp) 4224
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Vector typeWorm Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemultiple cloning site 3
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Alt nameMCS3
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SpeciesS. stercoralis (nematode)
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Insert Size (bp)20
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site MluI (not destroyed)
- 3′ cloning site AvrII (not destroyed)
- 5′ sequencing primer M13 forward (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
derived from pAJ04 with Mulitple cloning site 3 (MCS3).
See Fire Lab C. elegans Vector Kit 1995 Documetation.
PAPER:Int J Parasitol. 2006 May 31;36(6):671-9. Epub 2006 Feb 7 Successful transgenesis of the parasitic nematode Strongyloides stercoralis requires endogenous non-coding control elements.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAJ15 was a gift from James Lok (Addgene plasmid # 14917 ; http://n2t.net/addgene:14917 ; RRID:Addgene_14917) -
For your References section:
Strongyloides stercoralis: cell- and tissue-specific transgene expression and co-transformation with vector constructs incorporating a common multifunctional 3' UTR. Junio AB, Li X, Massey HC, Nolan TJ, Todd Lamitina S, Sundaram MV, Lok JB. Exp Parasitol. 2008 Feb . 118(2):253-65. 10.1016/j.exppara.2007.08.018 PubMed 17945217