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pAJ01
(Plasmid #14908)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14908 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPD95.75
  • Backbone manufacturer
    Andrew Fire
  • Backbone size w/o insert (bp) 4494
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    strongyloides stercoralis era-1 3' UTR
  • Alt name
    era-1 3' UTR
  • Species
    S. stercoralis (nematode)
  • Insert Size (bp)
    598
  • GenBank ID
    DQ333399

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EagI (not destroyed)
  • 5′ sequencing primer M13 forward
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Promoterless gfp expression construct with era-1 3' UTR.
See Fire Lab C. elegans Vector Kit 1995 Documetation.

PAPER:Int J Parasitol. 2006 May 31;36(6):671-9. Epub 2006 Feb 7 Successful transgenesis of the parasitic nematode Strongyloides stercoralis requires endogenous non-coding control elements.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAJ01 was a gift from James Lok (Addgene plasmid # 14908 ; http://n2t.net/addgene:14908 ; RRID:Addgene_14908)

  • For your References section:

    Strongyloides stercoralis: cell- and tissue-specific transgene expression and co-transformation with vector constructs incorporating a common multifunctional 3' UTR. Junio AB, Li X, Massey HC, Nolan TJ, Todd Lamitina S, Sundaram MV, Lok JB. Exp Parasitol. 2008 Feb . 118(2):253-65. 10.1016/j.exppara.2007.08.018 PubMed 17945217
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