HLA-A2/K^b
(Plasmid #14906)

• Depositing Lab: Linda Sherman
• Publication: Irwin et al J Exp Med. 1989 Oct 1. 170(4):1091-101.

Backbone

• Vector backbone: pSV2neo
• Backbone size w/o insert (bp): 5700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HLA-A2/K^b
• Alt name: HLA-A2.1
• Alt name: H-2K^b
• Alt name: HLA-A
• Species: H. sapiens (human)
• Insert Size (bp): 8200
• Entrez Gene: HLA-A (a.k.a. HLAA)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: BamHI (destroyed during cloning)
• 5′ sequencing primer: SV40pro-F

Resource Information

• A portion of this plasmid was derived from a plasmid made by: A2 from Harry Orr; K^b from Bruce Wallace
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

A Hind III-Bgl II fragment of the genomic clone of HLA-A2.1, which contained the genetic information coding for the leader sequence, alphal and alpha2 domains of the HLA-A2.1 molecule, was ligated to a Bam HI-Bam HI fragment excised from the Kb gene, which contained the alpha3, cytoplasmic, and transmembrane regions of the Kb molecule . The A2/Kb construct was purified and ligated into the Bam HI-Hind III sites of pSV2. The depositor notes that this contains the HLA-A2.1 Promoter, and was used for making the transgenic mouse in Vitiello A et.al.

This construct was originally described as conferring Neomycin resistance, but based on user feedback, we are no longer confident that the Neo-resistance gene is intact.