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Addgene

pGBW-m4046513
(Plasmid #149055)

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Item Catalog # Description Quantity Price (USD)
Plasmid 149055 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    AmpR; 2u URA3
  • Backbone size w/o insert (bp) 4078
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SARS-CoV-2 ORF3a protein
  • Species
    S. cerevisiae (budding yeast); Severe acute respiratory syndrome coronavirus 2
  • Insert Size (bp)
    821
  • Mutation
    Saccharomyces cerevisiae recode 1
  • Entrez Gene
    ORF3a (a.k.a. GU280_gp03)
  • Promoter pTEF1
  • Tag / Fusion Protein
    • Cleavable thrombin;MBP (C terminal on backbone)

Cloning Information

  • Cloning method Unknown

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    GinkgoBioworks

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

NCBI Reference Sequence: YP_009724391.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGBW-m4046513 was a gift from Ginkgo Bioworks & Benjie Chen (Addgene plasmid # 149055 ; http://n2t.net/addgene:149055 ; RRID:Addgene_149055)
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