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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 14904 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUC9
- Backbone size w/o insert (bp) 2700
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHLA-A2.1
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Alt nameHLA-A
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SpeciesH. sapiens (human)
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Insert Size (bp)5100
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Entrez GeneHLA-A (a.k.a. HLAA)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer M13R
- 3′ sequencing primer M13-F20 (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byHarry Orr
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
For more information, see Koller BH and Orr HT, J Immunol. 1985 Apr; 134(4):2727-33.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HLA-A2.1 was a gift from Linda Sherman (Addgene plasmid # 14904 ; http://n2t.net/addgene:14904 ; RRID:Addgene_14904) -
For your References section:
Species-restricted interactions between CD8 and the alpha 3 domain of class I influence the magnitude of the xenogeneic response. Irwin MJ, Heath WR, Sherman LA. J Exp Med. 1989 Oct 1. 170(4):1091-101. 10.1084/jem.170.4.1091 PubMed 2477484
Map uploaded by the depositor.
